rabbit anti mouse phosphorylated β catenin  (Cell Signaling Technology Inc)

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: The Protective Effects of Curcumin on Obesity-Related Glomerulopathy Are Associated with Inhibition of Wnt/ β -Catenin Signaling Activation in Podocytes

doi: 10.1155/2015/827472

Figure Lengend Snippet: Sequence of oligo used in the study.

Article Snippet: Rabbit anti-mouse phosphorylated β -catenin pAb (Cell Signaling Technology) , Ditto.

Techniques: Sequencing

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: The Protective Effects of Curcumin on Obesity-Related Glomerulopathy Are Associated with Inhibition of Wnt/ β -Catenin Signaling Activation in Podocytes

doi: 10.1155/2015/827472

Figure Lengend Snippet: Primary and secondary antibodies used in the study.

Article Snippet: Rabbit anti-mouse phosphorylated β -catenin pAb (Cell Signaling Technology) , Ditto.

Techniques:

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: The Protective Effects of Curcumin on Obesity-Related Glomerulopathy Are Associated with Inhibition of Wnt/ β -Catenin Signaling Activation in Podocytes

doi: 10.1155/2015/827472

Figure Lengend Snippet: Effects of curcumin on leptin-activated Wnt/ β -catenin signaling in cultured podocytes. Well-differentiated conditionally immortalized mouse podocytes were incubated in normal medium, medium containing 15 ng/mL leptin, medium containing 5 μ mol/L curcumin, or medium containing both 15 ng/mL leptin and 5 μ mol/L curcumin, respectively. (a) After 9 h, cells were collected and the mRNA expression levels of Wnt1, Wnt2b, Wnt6, and β -catenin were measured by relative quantitative real-time RT-PCR. (b) After 24 h, cells were lysed and total lysates were analyzed by Western blot assay with anti-phosphorylated β -catenin antibody and total β -catenin antibody, respectively. The relative phosphorylated β -catenin level was expressed as the phosphorylated β -catenin/total β -catenin ratio. Values are represented as mean ± SD ( n = 3). ∗ P < 0.05 versus control group and # P < 0.05 versus leptin alone group.

Article Snippet: Rabbit anti-mouse phosphorylated β -catenin pAb (Cell Signaling Technology) , Ditto.

Techniques: Cell Culture, Incubation, Expressing, Quantitative RT-PCR, Western Blot, Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: The Protective Effects of Curcumin on Obesity-Related Glomerulopathy Are Associated with Inhibition of Wnt/ β -Catenin Signaling Activation in Podocytes

doi: 10.1155/2015/827472

Figure Lengend Snippet: Effects of curcumin on Wnt/ β -catenin signaling in the kidney tissue of ORG mice. (a) Mice sacrificed at the end of 12th week. Total RNA was extracted from kidney cortex, and then the relative mRNA expression levels of Wnt1, Wnt2b, Wnt6, and β -catenin were measured by relative quantitative real-time RT-PCR. (b) Kidney cortex was lysed and total lysates were analyzed by Western blot assay with anti-phosphorylated β -catenin antibody and total β -catenin antibody, respectively. The relative phosphorylated β -catenin level was expressed as the phosphorylated β -catenin/total β -catenin ratio. Values are represented as mean ± SD ( n = 7). ∗ P < 0.05 versus control group and # P < 0.05 versus model group.

Article Snippet: Rabbit anti-mouse phosphorylated β -catenin pAb (Cell Signaling Technology) , Ditto.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: The Protective Effects of Curcumin on Obesity-Related Glomerulopathy Are Associated with Inhibition of Wnt/ β -Catenin Signaling Activation in Podocytes

doi: 10.1155/2015/827472

Figure Lengend Snippet: Effects of DKK1 on leptin-mediated podocyte injury. Well-differentiated conditionally immortalized mouse podocytes were incubated in normal media, media containing 15 ng/mL leptin and medium containing both 15 ng/mL leptin and 200 ng/mL DKK1, a well-known inhibitor of Wnt/ β -catenin signaling, respectively. (a) After 9 h, cells were collected and the mRNA expression levels of nephrin, podocin, podoplanin, and podocalyxin were measured by relative quantitative real-time RT-PCR. (b) After 24 h, cells were lysed and total lysates were analyzed by Western blot assay with antibodies of nephrin, podocin, podoplanin, podocalyxin, and β -actin, respectively. The relative protein level was expressed as the protein/ β -actin ratio. Values are represented as mean ± SD ( n = 3). ∗ P < 0.05 versus control group, ∗∗ P < 0.01 versus control group, and # P < 0.05 versus leptin alone group.

Article Snippet: Rabbit anti-mouse phosphorylated β -catenin pAb (Cell Signaling Technology) , Ditto.

Techniques: Incubation, Expressing, Quantitative RT-PCR, Western Blot, Control